Different Types of Cuvettes and Their Applications
UV spectroscopy, also called UV–visible spectrophotometry (UV–Vis), is a type of spectroscopy that looks at how light is absorbed or reflected in a part of the ultraviolet spectrum and the full visible spectrum that is right next to it.
This method is cheap and easy to use, so it is used a lot in a wide range of applied and basic fields. The only requirement is that the sample must be a chromophore, which means that it must absorb light in the UV-vis range.
Both fluorescence spectroscopy and absorption spectroscopy work well together. Along with the measurement wavelength, what is important is how the absorbance (A), transmittance (percent T), and reflectance (percent R) change over time.
Because glass and most plastics absorb UV light and cause interference, measurements in the ultraviolet region used to be done with uv vis quartz cuvettes.
There are now disposable plastic cuvettes made of certain polymers that are clear to ultraviolet light. Cuvettes made of glass, plastic, or quartz can all be used to measure longer wavelengths, like visible light.
Tandem cuvettes have a glass barrier that goes up about two-thirds of the way in the middle. This makes it possible to take measurements with two different solutions and then again when they are mixed. Most of the time, a 10mm cuvette is used for UV-VIS.